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Below, we describe a pharmacogenetics strategy which allows to selectively and reversibly inactivate LC neurons applying Designer Receptors Exclusively Activated by Designer Medicine (DREADD). We show which the expression from the hM4Di DREADD could be limited to noradrenergic LC neurons and that the quantity of LC inhibition is usually adjusted by adapting the dose of the particular DREADD activator deschloroclozapine (DCZ). In truth, even when significant doses (>0.3 mg/kg) induce an enormous inhibition of LC neurons and a transparent decrease in vigilance, lesser doses (> ×
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